AbstracIntroduction: Staph. aureus is a versatile pathogen capable of causing a wide variety of infections. With the development of resistance to all β-lactum antibiotics, treatment of these infections has become problematic. In recent years MRSA remains one of the most prevalent multidrug resistant organism causing healthcare associated infections. Aims and Objectives: To Study prevelance Nasal Colonization of MRSA in Health Care Workers of Bharati Hospital Sangali Maharashtra. Methodology: This was a Cross-sectional Study carried out in Health Care Workers of Bharati Hospital Sangali Maharashtra during the Period of January 2015 to January 2016. Over all 82-Doctor;31-Intern;57-Nurse;20-Attender;14-Laboratory personnel ;5-Others health workers were included into the study. Those workers who given consent wish to participate into study were included while those who did not given consent were excluded from the study. A Nasal Swab was collected from Participants with all aseptic precaution. From the collected samples Microscopy and Culture and Antibiotic Sensitivity testing were carried out using Kirby-Bauer’s disc diffusion method as per CLSI guidelines. Result: Majority of the Health workers Shown prevalence of S. aureus and MRSA was in Doctor i.e. 8.13% and1.43% followed by Nurse-4.30%and2.39%; Laboratory personnel-2.87% and0.47%; Intern-1.9% and0.95%;Attender-1.43% and0.95%; Others -0.95% and0.47%; Overall was 19.61% and 6.7% Respectively. Maximum resistant was found to Penicillin i.e. 70% followed by Erythromycin-40%; Cloxacillin-29.8%; Cefoxitin-21.9%; Cotrimoxazole-18.1 %;Gentamycin-16%; Ceftriaxone-10%; Teicoplanin-9.4%; Tetracycline-7.2%, Ciprofloxacin-3.1%; Clindamycin-3%; Amikacin-1% Intermediate Resistant to Amikacin and No resistant to Vancomycin was seen. Conclusion: Majority of the Health workers Shown prevalence of S. aureus and MRSA was in Doctor followed by Nurse, Laboratory personnel, Intern, Attender, Others but prevealence of MRSA found more in Nurses and Overall Maximum resistance to drugs were Penicillin, Erythromycin while there was no resistance found to Vancomycin and only intermediate resistance to Amikacin.

Keywords: MRSA, Health Care Workers, CLSI guidelines