Table of Content - Volume 17 Issue 2- February 2021
A retrospective study of seroprevalence of Hepatitis B virus infection among antenatal women in a tertiary care hospital, Ahmedabad
Rosy Parmar1, Asha Mandalia2*
1Associate Professor, 2Tutor, Department of Microbiology, GMERS Medical College, Sola, Ahmedabad, Gujrat, INDIA. Email: ashamandalia@gmail.com
Abstract Background: Type B hepatitis is the widest spread and most important type among all viral hepatitis. The spread of infection from mothers who are carriers of HBV to their babies appears to be an significant mode of transmission in China and Southeast Asia. The screening of Hepatitis B virus infection in antenatal women helps to prevent vertical transmission. This study was carried out to know the prevalence of hepatitis B virus in asymptomatic Antenatal women. Material and Method: All the blood samples were collected in sterile plain vaccutte in Antenatal care clinic and sent to Microbiology laboratory. Samples were then tested to detect HBsAg (viral marker) by Enzyme Linked Immunosorbent Assay (ELISA) and Immunochromatography ((rapid) technique. Result: In our study seroprevalence of hepatitis B virus in pregnant women was 0.50% of which the maximum patients were of age group 21-30 which is a child bearing age. Therefore screening of pregnant women for hepatitis B virus is necessary in order to identify those neonates at risk of transmission. Conclusion: As this viral infection is preventable through vaccination, early screening and creating awareness would be helpful to the community to reduce the number of Hepatitis B virus infection in new born and improve health of community. Key words: Hepatitis B virus, antenatal screening, ELISA, immunochromatography.
INTRODUCTION Hepatitis B virus infection is a serious global public health problem.2 Hepatitis B virus is a double stranded DNA virus composed of a nucleocapsid core (HBcAg), surrounded by an outer lipoprotein coat (also called envelope) containing the surface antigen (HBsAg).3 It is a type of DNA virus belonging to the family Hepadnaviridae.4 The virus was first discovered as ‘Australian antigen’ and later named Hepatitis B surface Antigen (HBsAg) in the blood of patient.5 Hepatitis B envelope Antigen (HBeAg) was discovered later as a marker for patients at a high risk for transmission of the disease.6,7 More than a one third of global population is estimated to be infected by HBV. About quarter of them become HBV carriers8. Worldwide about 1 million deaths occur each year due to chronic form of disease9. The sera of infected patients may contain as many as 1010 infectious Virion per ml3. The natural reservoir of HBV is man. Hepatitis B virus attacks liver cells and can lead to liver failure, cirrhosis or cancer of liver later in life. The asymptomatic HBsAg positive pregnant women can be identified by detecting HBsAg in their blood. Transmission can occur at any stage; in utero, during delivery (maximum risk) and during breast feeding. Risk is maximum if mother is HBeAg positive.1 If mother is positive for HBsAg) then immediate care of baby to be taken in form of immunoglobulin and vaccination. Antenatal screening will also help in preventing further transmission to her spouse by taking due precaution during sexual contact.10
OBJECTIVE To determine seroprevalence of Hepatitis B infection in antenatal women and to prevent vertical transmission to the baby.
MATERIAL AND METHODS For the present study 4131 pregnant women visiting antenatal care clinic in the Department of Obstetrics and Gynecology, GMERS medical college and hospital, Sola were studied for detection of HBsAg between January 2020 to December 2020. The approval was obtained from the institute research council and ethics committee of sola civil hospital, Ahmedabad prior to the commencement of this study. Every pregnant woman coming for antenatal care at the study location was screened for Hepatitis B virus according to national guidelines.11 All pregnant women were evaluated for serum HBsAg using ELISA (Merilisa kit) and Rapid immunographic test. (Reckon Kit). Study design This retrospective study was conducted to determine the seroprevalence of the HBsAg among the women visiting antenatal care clinic at GMERS medical college and hospital, Sola, Ahmedabad. Study population The study population was pregnant women attending antenatal clinic in GMERS medical college and hospital. Study duration The study was conducted from 1st January 2020 to 31st December 2020 Inclusion criteria All the pregnant women who visited for antenatal care at the institute Exclusion criteria Women who were not pregnant.
OBSERVATION AND RESULT Total 4131 antenatal women were screened for Hepatitis B Surface Antigen in GMERS medical college and hospital, sola, Ahmedabad form January 2020 to December 2020. A total of 21 cases were found to have HBsAg positive out of 4131 who underwent testing, indicating a prevalence rate of 0.50%. Such low prevalence is suggestive of increased awareness of people towards vaccination and routine antenatal screening. The mean age of HBsAg positive pregnant women was 26.23 years. Four subjects (19%) were from rural area while 17 (81%) were residing in urban area. None of these antenatal women had HBV-HCV or HBV-HIV co-infection. Most common age group with HBV infection was 25 – 30 years, with 21 subjects indicating a prevalence rate of 0.50%.
TABLE: 1 Seroprevalence based on age group of Antenatal women
TABLE: 2 Seroprevalence based on domicile among HBV positive antenatal women
DISCUSSION Seroprevalence of hepatitis B virus in antenatal women was 0.50% in our study. We have also compared some other studies from different parts of India showing different prevalence of Hepatitis B virus in antenatal women.
Our study showed Hepatitis B prevalence rate 0.50% out of 4131 samples in antenatal women which is lower than the previous studies mentioned in the above table which might be due to increase awareness in form of increase number of screening in antenatal population, due to availability of an effective vaccine against Hepatitis B and awareness amongst population for use of this vaccine.
CONCLUSION The risk of chronic infection in Hepatitis B is more if the infection is acquired in early age. The risk of chronicity is >90% in newborn. Neonates who acquire infection from their mother during pregnancy have higher chances of becoming chronic carrier. Such babies develop chronic liver disease at a younger age and represent the most important reservoir of infection in the community. Thus early screening and detection helps in prevention of vertical transmission from mother to foetus and decreases overall carrier rate. Perinatal transmission can be prevented by administration of HBIG to babies within 48 hours of birth. The risk of transmission increases if mother is Hepatitis B envelope Antigen (HBeAg) positive. So, along with HBsAg detection, HBeAg detection should be included in antenatal screening. To further reduce the prevalence, awareness campaign should be run among community regarding modes of transmission, screening and availability of effective vaccine for hepatitis B virus.
REFERENCES
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